OH - HIC

OH (hydroxy)

 Uncharged (neutral) ligand

 Working range, pH 2–10

 Retains very large solutes (virus particles, VLP, vesicles) in the presence of precipitating salts or polyethylene glycol, and elutes them with high resolution in order of increasing size

 Elution with descending salt or polyethylene glycol

 Sanitizable with 1 M NaOH